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Clinical Chemistry 37: 701-706, 1991;
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Clinical Chemistry, Vol 37, 701-706, Copyright © 1991 by American Association for Clinical Chemistry

Single procedure for detection, confirmation, and quantification of benzodiazepines in serum by liquid chromatography with photodiode-array detection

PR Puopolo, ME Pothier, SA Volpicelli and JG Flood
Chemistry Laboratory, Massachusetts General Hospital, Boston 02114.

We developed a reversed-phase chromatographic procedure for detecting benzodiazepines and other drugs in serum. A liquid-liquid extraction step with hexane/ethyl acetate isolates the drugs from serum; absolute recoveries are generally greater than 85%. Reconstituted extracts are chromatographed on a 4-microns (particle size) C18 column; 14 drugs and an internal standard (flunitrazepam) are separated in 8 min. Peak detection, purity checking, and identification are performed with a computerized photodiode-array detector. Run-to-run imprecision (CV) for many benzodiazepines is less than 3%. In a study of 126 specimens from Emergency Department patients, the procedure showed excellent agreement with a gas-chromatographic method involving either mass-spectrometric or flame-ionization detection. This single procedure provides rapid and accurate detection, quantification, and confirmation of benzodiazepines in serum.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
C.-K. Lai, T. Lee, K.-M. Au, and A. Y.-W. Chan
Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection
Clin. Chem., February 1, 1997; 43(2): 312 - 325.
[Abstract] [Full Text] [PDF]


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Copyright © 1991 by the American Association for Clinical Chemistry.