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Clinical Chemistry 37: 709-714, 1991;
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Clinical Chemistry, Vol 37, 709-714, Copyright © 1991 by American Association for Clinical Chemistry

Blood-collection device for trace and ultra-trace metal specimens evaluated

TP Moyer, GV Mussmann and DE Nixon
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.

We evaluated the evacuated phlebotomy tube designed specifically for trace metal analysis by Sherwood Medical Co. Pools of human serum containing known concentrations of aluminum, arsenic, calcium, cadmium, copper, chromium, iron, lead, magnesium, manganese, mercury, selenium, and zinc were exposed to the tube and rubber stopper for defined periods ranging from 5 min to 24 h. Analysis for each element was performed in a randomized fashion under rigidly controlled conditions by use of standard electrothermal atomization atomic absorption spectroscopy, inductively coupled plasma atomic emission spectroscopy, and cold vapor atomic absorption spectrometry. In addition, for comparative purposes, we collected blood samples from normal volunteers by use of ultra-clean polystyrene phlebotomy syringes as well as standard evacuated phlebotomy tubes. We conclude that, except for lead, there was no significant contribution of any trace element studied from the evaluated tube and stopper to the serum. Because whole blood is the usual specimen for lead testing, the observation of a trace amount of lead in this tube designed for serum collection is trivial.




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Copyright © 1991 by the American Association for Clinical Chemistry.