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Clinical Chemistry 37: 832-837, 1991;
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Clinical Chemistry, Vol 37, 832-837, Copyright © 1991 by American Association for Clinical Chemistry

Versatile, non-clotting-based heparin assay requiring no instrumentation

W Chen and VC Yang
College of Pharmacy, University of Michigan, Ann Arbor 48109-1065.

A non-clotting-based heparin assay requiring no instrumentation has been developed by attaching protamine (a heparin antidote) to a porous filter paper strip and monitoring the subsequent capillary migration of heparin sample through the paper. The area of paper to which heparin adsorbed, which is proportional to the concentration of heparin in the sample, is made visible by spraying Methylene Blue NNX solution onto the paper strip when the sample reservoir has emptied. The dye interacts with heparin to cause a metachromatic shift of the dye's absorption maximum from blue to purple. Heparin concentrations in the samples are estimated according to the length of the purple region on the paper strip. Heparin in plasma (2 to 35 int. units/mL) can be differentiated in approximately 6 min. The plasma heparin values derived by the new method agree reasonably well with those obtained by conventional assays (r greater than 0.99, n = 48).


The following articles in journals at HighWire Press have cited this article:


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 CLIN APPL THROMB HEMOSTHome page
C. H. Mielke JR, C. M. Starr, J. C. Klock, D. Devereaux, M. R. Mielke, D. E. Baker, L. Broemeling, M. Wacksman, J. R. White JR, S. A. Oliver, et al.
Direct Measurement of Unfractionated Heparin Using a Biochemical Assay
Clinical and Applied Thrombosis/Hemostasis, October 1, 1999; 5(4): 267 - 276.
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Copyright © 1991 by the American Association for Clinical Chemistry.