Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 37: 868-874, 1991;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nanjee, M. N.
Right arrow Articles by Miller, N. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nanjee, M. N.
Right arrow Articles by Miller, N. E.

Clinical Chemistry, Vol 37, 868-874, Copyright © 1991 by American Association for Clinical Chemistry

Enzymatic fluorometric procedure for phospholipid quantification with an automated microtiter plate fluorometer

MN Nanjee, AK Gebre and NE Miller
Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, NC 27103.

We describe a new enzymatic assay for phospholipids that is rapid, sensitive, convenient, and inexpensive. The method is based on the fluorometric detection of H2O2, generated by the choline oxidase- catalyzed oxidation of choline, after liberation of choline from phospholipids by phospholipase D. Significant advantages over existing methods are that the entire reaction sequence can take place in a single vessel, a 12 x 8 well microtiter plate, and that the fluorescence intensity can be measured automatically with a Fluoroskan II (Labsystems Oy) detector. Extracting samples with organic solvents is unnecessary, although the method can be applied to extracts in isopropanol. The assay is approximately 60-fold more sensitive and has a limit of detection eightfold lower than currently available enzymatic colorimetric methods. Including solvent blanks, eight standards, and three quality-control pools, 34 samples can be pipetted and assayed in duplicate in 60 min. Results obtained by this procedure for total phospholipid choline in lipoproteins in primate plasma agreed well with those obtained for inorganic phosphorus by the Bartlett acid-digestion procedure.


The following articles in journals at HighWire Press have cited this article:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y.-H. M. Chan, B. van Lengerich, and S. G. Boxer
Inaugural Article: Effects of linker sequences on vesicle fusion mediated by lipid-anchored DNA oligonucleotides
PNAS, January 27, 2009; 106(4): 979 - 984.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Ravasio, A. Cruz, J. Perez-Gil, and T. Haller
High-throughput evaluation of pulmonary surfactant adsorption and surface film formation
J. Lipid Res., November 1, 2008; 49(11): 2479 - 2488.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Garcia-Verdugo, A. Ravasio, E. G. de Paco, M. Synguelakis, N. Ivanova, J. Kanellopoulos, and T. Haller
Long-term exposure to LPS enhances the rate of stimulated exocytosis and surfactant secretion in alveolar type II cells and upregulates P2Y2 receptor expression
Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L708 - L717.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the American Association for Clinical Chemistry.