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Clinical Chemistry 37: 1173-1177, 1991;
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Clinical Chemistry, Vol 37, 1173-1177, Copyright © 1991 by American Association for Clinical Chemistry

Isolation and characterization of zinc coproporphyrin I: a major fluorescent component in meconium

K Horiuchi, K Adachi, Y Fujise, H Naruse, K Sumimoto, N Kanayama and T Terao
Department of Chemistry, Hamamatsu University School of Medicine, Japan.

We isolated a substance from an organic extract of meconium (in neutral pH) that exhibited a porphyrin-like fluorescence peak at 580 nm on excitation at 405 nm and deduced its structure. Spectrometric data suggest that the substance is not free coproporphyrin I or free coproporphyrin III, but is a chelate of coproporphyrin I with Zn2+. We also detected a chelate of coproporphyrin III with Zn2+ by HPLC. These substances may be useful as new indicators of the presence of meconium.


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