Clinical Chemistry
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Clinical Chemistry 37: 1178-1184, 1991;
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Clinical Chemistry, Vol 37, 1178-1184, Copyright © 1991 by American Association for Clinical Chemistry

Rapid and specific enzyme immunoassay of serotonin

J Chauveau, V Fert, AM Morel and MA Delaage
Immunotech, Marseille, France.

A new, highly sensitive enzyme immunoassay (EIA) of serotonin (5HT) is described. The assay is based on the competition between N-succinyl- glycinamide-serotonin (N-SGA-5HT, obtained by acylation of the 5HT in the sample to be assayed) and an enzymic tracer, N-succinyl-5HT- acetylcholinesterase, for binding to rabbit polyclonal antibody coated onto the wells of microtiter plates. The antibody is directed against an immunogen obtained by coupling N-succinyl-5HT to glycyl-bovine serum albumin. The EIA permits the accurate measurement of as little serotonin as 0.5 nmol/L (1.8 pg per well) in blood, plasma, serum, cerebrospinal fluid, urine, platelets, and other tissues, with no significant cross-reactivity with other compounds. The results obtained correlate well with those obtained by HPLC after extraction. The assay has the advantage of permitting the measurement of 5HT in up to 500 samples in as little as 3 h.




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Copyright © 1991 by the American Association for Clinical Chemistry.