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Clinical Chemistry 37: 1283-1286, 1991;
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Clinical Chemistry, Vol 37, 1283-1286, Copyright © 1991 by American Association for Clinical Chemistry

Aluminum phthalocyanine-streptavidin: new, sensitive fluorescent tracer for immunoassay

TA Kelly, CA Hunter, DC Schindele and BV Pepich
Ultra Diagnostics Corp., Seattle, WA 98105.

A new fluorescent reagent based on aluminum phthalocyanine has been developed for general application to immunoassay. This highly sensitive fluorophore, Ultralite 680, may be covalently attached to a variety of biological entities for use as a tracer. We report the use of an Ultralite 680-streptavidin-digoxin conjugate as a tracer in a direct, competitive, heterogeneous, fluorescent immunoassay of digoxin. The assay is performed manually with 50 microL of serum and no sample pre- treatment. Primary anti-digoxin antibody is incubated with a serum sample containing digoxin and Ultralite 680-streptavidin-digoxin. Bound and free tracer are separated, and the bound fluorescence is released and quantified with a commercially available spectrofluorometer. Assay precision was good (CV = 7.05% at a digoxin concentration of 2.00 micrograms/L) and results by our method correlated well with those by a conventional RIA (r = 0.9650, n = 24).


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 Clin. Chem.Home page
A. Scholer, J. Boecker, U. Engelmayer, K. Feldmann, D. Hannak, R. Kattermann, M. Oellerich, H. Raith, H. Schlebusch, H. Wieland, et al.
Comparability of a new turbidimetric digoxin test with other immunochemical tests and with HPLC—a multicenter evaluation
Clin. Chem., January 1, 1997; 43(1): 92 - 99.
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Copyright © 1991 by the American Association for Clinical Chemistry.