Clinical Chemistry
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Clinical Chemistry 37: 1370-1374, 1991;
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Clinical Chemistry, Vol 37, 1370-1374, Copyright © 1991 by American Association for Clinical Chemistry

Measurement of the ratio of lecithin to sphingomyelin in amniotic fluid by fast atom bombardment mass spectrometry

A Shimizu, Y Ashida and F Fujiwara
Osaka Medical Center, Japan.

We describe a fast atom bombardment mass spectrometric method to determine the ratio of lecithin to sphingomyelin in amniotic fluid. Between m/z 400 and 1000, amniotic fluid extracts showed clear peaks for only sphingomyelin and lecithin. The molecular species of fatty acid in the phospholipids were analyzed quantitatively and quickly in a small volume of the extracts. The dipalmitoyllecithin/palmitoylsphingomelin ratio, determined by the proposed method, showed a reasonable correlation to the lecithin/sphingomyelin ratio measured by thin-layer chromatography. This method allows rapid and sensitive measurement of these phospholipids.


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J. Lipid Res.Home page
R. L. Shaner, J. C. Allegood, H. Park, E. Wang, S. Kelly, C. A. Haynes, M. C. Sullards, and A. H. Merrill Jr.
Quantitative analysis of sphingolipids for lipidomics using triple quadrupole and quadrupole linear ion trap mass spectrometers
J. Lipid Res., August 1, 2009; 50(8): 1692 - 1707.
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Copyright © 1991 by the American Association for Clinical Chemistry.