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Clinical Chemistry 37: 1370-1374, 1991;
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Clinical Chemistry, Vol 37, 1370-1374, Copyright © 1991 by American Association for Clinical Chemistry

Measurement of the ratio of lecithin to sphingomyelin in amniotic fluid by fast atom bombardment mass spectrometry

A Shimizu, Y Ashida and F Fujiwara
Osaka Medical Center, Japan.

We describe a fast atom bombardment mass spectrometric method to determine the ratio of lecithin to sphingomyelin in amniotic fluid. Between m/z 400 and 1000, amniotic fluid extracts showed clear peaks for only sphingomyelin and lecithin. The molecular species of fatty acid in the phospholipids were analyzed quantitatively and quickly in a small volume of the extracts. The dipalmitoyllecithin/palmitoylsphingomelin ratio, determined by the proposed method, showed a reasonable correlation to the lecithin/sphingomyelin ratio measured by thin-layer chromatography. This method allows rapid and sensitive measurement of these phospholipids.




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Copyright © 1991 by the American Association for Clinical Chemistry.