Clinical Chemistry
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Clinical Chemistry 37: 1436-1441, 1991;
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Clinical Chemistry, Vol 37, 1436-1441, Copyright © 1991 by American Association for Clinical Chemistry

Effects of storage time and temperature on urinary enzymes

E Matteucci, G Gregori, L Pellegrini, R Navalesi and O Giampietro
Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Universita degli Studi di Pisa, Italy.

We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC 1.1.1.27; gamma-glutamyltransferase, GGT, EC 2.3.2.2; and alkaline phosphatase, AP, EC 3.1.3.1) in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrations. Short-term (24 h) storage time at room temperature or 4 degree C does not affect urinary enzyme activity. A few days of freezing, at -20 or -70 degrees C, dramatically lowers LDH and AP values; GGT is partially preserved only at -70 degrees C, if the sample has been previously centrifuged. Urinary enzymes investigated in this study are extremely labile at low temperatures.


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