Clinical Chemistry
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Clinical Chemistry 38: 11-18, 1992;
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Clinical Chemistry, Vol 38, 11-18, Copyright © 1992 by American Association for Clinical Chemistry

One-step, labeled-antibody assay for measuring free thyroxin. I. Assay development and validation

ND Christofides, CP Sheehan and JE Midgley
Amerlite Diagnostics Ltd., Cardiff Laboratories, Whitchurch, U.K.

We describe a one-step, labeled-antibody radioassay for measuring free thyroxin (FT4) in serum or plasma, based on a novel principle. FT4 in the sample competes with a gross molar excess (over antibody) of a cross-reactant (L-triiodothyronine, T3), chemically coupled to magnetizable polymer particles, for binding to avid 125l-labeled monoclonal anti-thyroxin antibodies. As in conventional immunoassays, 125I counts bound to the solid phase (T3-magnetizable particles) are inversely proportional to sample FT4 concentration. We demonstrate here the development and technical validity of this new method.


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J. E.M. Midgley and N. D. Christofides
Point: Legitimate and Illegitimate Tests of Free-Analyte Assay Function
Clin. Chem., March 1, 2009; 55(3): 439 - 441.
[Full Text] [PDF]

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J. E.M. Midgley
Direct and Indirect Free Thyroxine Assay Methods: Theory and Practice
Clin. Chem., August 1, 2001; 47(8): 1353 - 1363.
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Copyright © 1992 by the American Association for Clinical Chemistry.