Clinical Chemistry
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Clinical Chemistry 38: 119-122, 1992;
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Clinical Chemistry, Vol 38, 119-122, Copyright © 1992 by American Association for Clinical Chemistry

Elimination of matrix effects in electrothermal atomic absorption spectrophotometric determinations of bismuth in serum and urine

S Dean, PJ Tscherwonyi and WJ Riley
Department of Biochemistry, Royal Perth Hospital, Western Australia.

A sensitive and precise electrothermal atomic absorption spectrophotometric method for determining bismuth concentration is described. Protein precipitation and the use of a palladium modifier reduce the problems of foaming and permit the use of a higher ashing temperature. The detection limit of the assay is 0.9 nmol/L. Total CVs (intra- and interassay) for serum ranged from 3.5% to 15.1% and for urine from 4.8% to 14.5% at concentrations of 60.0 and 6.0 nmol/L, respectively. Analytical recoveries of bismuth added to serum and urine were 102% and 103% over the same range. The method is robust and reproducible and can be accurately calibrated with aqueous standards.




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Copyright © 1992 by the American Association for Clinical Chemistry.