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Clinical Chemistry 38: 1963-1967, 1992;
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Clinical Chemistry, Vol 38, 1963-1967, Copyright © 1992 by American Association for Clinical Chemistry

Direct, simplified, and sensitive assay of angiotensin II in plasma extracts performed with a high-affinity monoclonal antibody

D Simon, B Romestand, H Huang, G Badouaille, JA Fehrentz, B Pau, J Marchand and P Corvol
Sanofi Recherche, Montepellier, France.

A very simple, fast, and sensitive RIA of angiotensin (Ang) II has been developed, based on a monoclonal antibody with high affinity and specificity, making possible the direct measurement of circulating Ang II in human plasma after solid-phase extraction. The purified monoclonal antibody 4D8 has an association constant of 1.3 x 10(11) L/mol with Ang II and a cross-reactivity of < 1% for Ang I. The assay can detect as little as 0.8 fmol of Ang II in 2 mL of plasma and is not influenced by the presence of Ang I. Analytical recoveries between 112% and 116% were obtained for Ang II added to human plasma at physiological concentrations. Comparison of the RIA with a reversed- phase, high-performance liquid chromatographic method followed by RIA to measure Ang II in human plasma samples from normal and hypertensive subjects--and from normotensive subjects before and after an acute inhibition of angiotensin-converting enzyme with captopril (50 mg)-- showed a high degree of correlation (r2 = 0.93) between the two methods.


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