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Clinical Chemistry 38: 2013-2018, 1992;
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Clinical Chemistry, Vol 38, 2013-2018, Copyright © 1992 by American Association for Clinical Chemistry

Determination of fetal hemoglobin by time-resolved immunofluorometric assay

U Turpeinen and UH Stenman
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.

We have developed a "sandwich"-type time-resolved immunofluorometric assay (IFMA) for fetal hemoglobin (HbF) in hemolysates from adults and newborns, amniotic fluid, and plasma, based on a polyclonal and a monoclonal antibody against human fetal hemoglobin. Microtiter wells are coated with polyclonal capture antibody, and the gamma-chain- specific monoclonal tracer antibody is labeled with a europium chelate. In a simple and fast assay procedure, prediluted hemolysates are incubated in the microtiter wells first with capture antibody for 1 h and, after washing, for 1 h with tracer antibody. The wells are washed and the fluorescence of europium is measured. The mean analytical recovery is 102% and results by IFMA agreed well with values obtained by high-performance liquid chromatography. The analytical range of IFMA is large and well suited for clinical purposes. The detection limit of the assay is 0.2 microgram/L and the measuring range extends to 500 micrograms/L.


The following articles in journals at HighWire Press have cited this article:


Home page
 Obstet GynecolHome page
P.-L. KUO and H.-R. GUO
Nucleated Red Blood Cells in Maternal Blood During Pregnancy
Obstet. Gynecol., September 1, 1999; 94(3): 464 - 468.
[Abstract] [Full Text] [PDF]


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