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Clinical Chemistry 38: 2089-2092, 1992;
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Clinical Chemistry, Vol 38, 2089-2092, Copyright © 1992 by American Association for Clinical Chemistry

Nonimmunological assay of urinary albumin based on laser-induced fluorescence

MA Kessler, MR Hubmann, BA Dremel and OS Wolfbeis
Analytical Division, Karl-Franzens University, Graz, Austria.

We describe the first nonimmunological assay of albumin in urine with a detection limit of 1 mg/L. The method is simple, rapid, and accurate. It is based on the probe Albumin Blue 670, which becomes highly fluorescent on binding to albumin. An inexpensive diode laser was used as the light source for measurement of laser-induced fluorescence. The assay was coupled to a flow-injection analysis system capable of running 20 samples per hour. The working range was 1-100 mg/L, which covered albumin concentrations found in nonpathological urine and in urine with slightly increased albumin. This range makes prediction of nephropathy possible at an early stage. Other serum proteins and hemoglobin do not interfere. The coefficients of variation were < 4% and < 7% within one day and from day to day, respectively. A correlation coefficient of 0.990 (n = 100) was obtained for comparison with the Behring nephelometric assay.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
S. Choi, E. Y. Choi, H. S. Kim, and S. W. Oh
On-Site Quantification of Human Urinary Albumin by a Fluorescence Immunoassay
Clin. Chem., June 1, 2004; 50(6): 1052 - 1055.
[Full Text] [PDF]

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M. A. Kessler, A. Meinitzer, W. Petek, and O. S. Wolfbeis
Microalbuminuria and borderline-increased albumin excretion determined with a centrifugal analyzer and the Albumin Blue 580 fluorescence assay
Clin. Chem., June 1, 1997; 43(6): 996 - 1002.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Association for Clinical Chemistry.