Clinical Chemistry
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Clinical Chemistry 38: 2365-2371, 1992;
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Clinical Chemistry, Vol 38, 2365-2371, Copyright © 1992 by American Association for Clinical Chemistry

Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material

F Schiele, J Muller, E Colinet, G Siest, P Arzoglou, H Brettschneider, DH Calam, F Ceriotti, G Ferard and J Frei
Laboratoire du Centre de Medecine Preventive et Centre du Medicament, UA CNRS No 597, Nancy, France.

We present the results of a study on performance of a reference material for alanine aminotransferase (ALT, EC 2.6.1.2) and the corresponding IFCC-approved method in an interlaboratory trial involving 13 laboratories. The ALT material was partly purified from pig heart (specific activity, 150 kU/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (EC 2.6.1.1). The partly purified ALT was lyophilized in a triethanolamine-buffered matrix, pH 6.4, containing bovine serum albumin and saccharose. Under these conditions, the predicted yearly loss of activity was 0.02% at 4 degrees C and < 0.01% at -20 degrees C. The final blank-corrected results of the accepted set of data gave a mean (SD) of 128.5 (5.1) U/L. The among-laboratory SD was 4.6 U/L and the within-laboratory SD was 2.0 U/L. The certified ALT catalytic concentration in the reconstituted material was 129 U/L with a 0.95 confidence interval of +/- 4 U/L.




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Copyright © 1992 by the American Association for Clinical Chemistry.