Clinical Chemistry
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Clinical Chemistry 38: 2387-2391, 1992;
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Clinical Chemistry, Vol 38, 2387-2391, Copyright © 1992 by American Association for Clinical Chemistry

Apolipoprotein B quantified by particle-concentration fluorescence immunoassay

BJ Hallaway, A Rastogi and BA Kottke
Atherosclerosis Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905.

We have developed a particle-concentration fluorescence immunoassay (PCFIA) for estimating apolipoprotein (apo) B concentrations in plasma. A two-step antigen-detection system with a polyclonal antibody to apo B bound to carboxyl-polystyrene particles binds the antigen, and a fluorescein-labeled monoclonal antibody detects the bound apo B. Narrow- cut low-density lipoproteins (d = 1.03-1.05 kg/L) were used as the primary standard. The assay compares well with the enzyme-linked immunosorbent assay. The PCFIA gives parallel responses with low- density lipoprotein, very-low-density lipoproteins, and plasma samples, and can be fully automated and completed in 3 h. In a pilot study of patients with diabetes, coronary artery disease (CAD), or both, we found statistically significant differences in apo B concentrations for patients with both CAD and diabetes compared with those for patients with diabetes alone or for control subjects (P < 0.01).




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Copyright © 1992 by the American Association for Clinical Chemistry.