Clinical Chemistry
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Clinical Chemistry 38: 2392-2395, 1992;
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Clinical Chemistry, Vol 38, 2392-2395, Copyright © 1992 by American Association for Clinical Chemistry

Use of synthetic H disaccharides as acceptors for detecting activities of UDP-GalNAc:Fuc alpha 1-->2Gal beta-R alpha 1-->3-N- acetylgalactosaminyltransferase in plasma samples from blood group A subgroups

S Yazawa, A Takeya, O Hosomi, T Nakajima, N Shimoda, H Ohi, T Tachikawa, CF Piscorz, RK Jain and KL Matta
Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.

Concentrations of blood group A-specified alpha(1-->3)-N- acetylgalactosaminyltransferase (A enzyme) were measured in human plasma of blood groups A1, A2, and A3 by using chemically synthesized H disaccharides and H type 1 and type 2 trisaccharides attached to hydrophobic aglycones as acceptors. When the trisaccharides were used as acceptors, enzyme activities were reduced in samples from A2 and A3. However, the H disaccharides were shown to be good acceptors even for enzymes from A2 and A3, and no significant difference in enzyme concentration was detected in any of the plasma tested.


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S. Yazawa, T. Nishimura, M. Ide, T. Asao, A. Okamura, S. Tanaka, I. Takai, Y. Yagihashi, A. R. Saniabadi, and N. Kochibe
Tumor-related expression of {alpha}1,2fucosylated antigens on colorectal carcinoma cells and its suppression by cell-mediated priming using sugar acceptors for {alpha}1,2fucosyltransferase
Glycobiology, September 1, 2002; 12(9): 545 - 553.
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