Clinical Chemistry
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Clinical Chemistry 38: 2401-2404, 1992;
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Clinical Chemistry, Vol 38, 2401-2404, Copyright © 1992 by American Association for Clinical Chemistry

Quantitative determination of methemoglobin by measuring the solvent- water proton-nuclear magnetic resonance relaxation rate

S Aime, W Dastru, M Fasano, A Arnelli, M Castagnola, B Giardina and P Ascenzi
Dipartimento di Chimica Inorganica, Universita di Torino, Italy.

We report a new method for the quantitative determination of human methemoglobin (metHb) based on the measurement of the solvent-water proton-nuclear magnetic resonance (NMR) relaxation rate R1 [normalized to 1 mmol/L hemoglobin (Hb) concentration]. MetHb (%) is estimated from the linear dependence of R1 on the metHb concentration, taking into account the simple relationship [MetHb] = [(R1 - R1HbO2)/(R1metHb - R1HbO2)].100, where R1HbO2 and R1metHb are values for the solvent-water relaxation rate of standard 1.0 mmol/L solutions of the oxygenated derivative of human hemoglobin (HbO2) and of metHb, respectively. The minimum metHb that may be determined from the analysis of the experimental data is 0.5 +/- 0.4%.




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Copyright © 1992 by the American Association for Clinical Chemistry.