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Clinical Chemistry 38: 2439-2443, 1992;
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Clinical Chemistry, Vol 38, 2439-2443, Copyright © 1992 by American Association for Clinical Chemistry

Direct determination of zinc in serum by Zeeman atomic absorption spectrometry with a graphite furnace

PC D'Haese, LV Lamberts, AO Vanheule and ME De Broe
Department of Nephrology-Hypertension, University Hospital Antwerp, University of Antwerp, Edegem, Belgium.

We developed a precise and accurate graphite furnace atomic absorption spectrometric method for the direct determination of zinc in serum. Serum samples are analyzed after 20-fold dilution with water of ultrapure analytical grade. No other reagent is used, from the moment of sampling until measurement. During atomization, the argon flow is kept at 150 mL/min instead of gas stop, to decrease the sensitivity and thus allow lower dilution ratios. Zinc concentrations are determined against a serum-matched calibration curve. Graphite tubes are uncoated and no L'vov platform is used. Between-run CVs were 5.9%, 3.5%, and 1.9% for serum zinc concentrations of 0.93, 1.15, and 1.43 mg/L, respectively. The characteristic mass was 9 pg, and the detection limit (meanblank + 3SDblank) was 0.060 mg/L.


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