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Clinical Chemistry 38: 2479-2483, 1992;
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Clinical Chemistry, Vol 38, 2479-2483, Copyright © 1992 by American Association for Clinical Chemistry

Arsenic analysis II: rapid separation and quantification of inorganic arsenic plus metabolites and arsenobetaine from urine

DE Nixon and TP Moyer
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.

We describe the rapid separation of inorganic arsenic plus metabolites from arsenobetaine or seafood arsenic in urine. Traditional, high- pressure liquid chromatography is replaced by disposable silica-based cation-exchange cartridges for this separation. Both fractions are quickly separated and collected for analysis by atomic absorption spectrophotometry. Analytical recovery of both fractions is > or = 95%, with an overall precision (CV) ranging from 1.6% to 6.4%. Using this method, we correctly identified the sources of arsenic exposure, whether of inorganic or seafood origin, in 11 urine specimens supplied by the Centre de Toxicologie du Quebec.




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Copyright © 1992 by the American Association for Clinical Chemistry.