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Clinical Chemistry 38: 2517-2525, 1992;
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Clinical Chemistry, Vol 38, 2517-2525, Copyright © 1992 by American Association for Clinical Chemistry

Phospholipase resistance of the glycosyl-phosphatidylinositol membrane anchor on human alkaline phosphatase

YW Wong and MG Low
Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons of Columbia University, New York, NY 10032.

Alkaline phosphatase (ALP) is attached to the cell surface in mammalian tissues via a glycosyl-phosphatidylinositol (GPI) anchor and can be released from the membrane by GPI-specific phospholipases. In a range of cultured human cell lines, however, the sensitivity of ALP to phospholipases was observed to be variable in magnitude (approximately 20-90%). The mechanism of phospholipase resistance was explored with phospholipases of different bond specificities. The results suggest that phospholipase resistance is the result of acylation of the inositol ring in the GPI anchor. The occurrence of phospholipase- resistant forms of ALP may have important implications for the in vivo release and disposition of plasma ALP.


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Fatty Acid Remodeling of GPI-anchored Proteins Is Required for Their Raft Association
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M. Ferguson
The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research
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