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Clinical Chemistry 38: 223-226, 1992;
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Clinical Chemistry, Vol 38, 223-226, Copyright © 1992 by American Association for Clinical Chemistry

alpha-Glucosidase and N-acetyl-beta-D-glucosaminidase isoenzymes in serum [published erratum appears in Clin Chem 1993 Feb;39(2):371]

P Coma, L Gomez-Chacon, B Garcia-Serrano, E Fernandez and MA Ortiz-Apodaca
Laboratorio de Bioquimica, Hospital Virgen de la Salud, Toledo, Spain.

Using different conditions for incubation and fluorometry with 4- methylumbelliferylglycosides as substrates, we demonstrated the presence of acid alpha-glucosidase, "renal" alpha-glucosidase, N-acetyl- beta-D-glucosaminidase A, and N-acetyl-beta-D-glucosaminidase B in freshly drawn normal human serum. The acid alpha-glucosidase enzymatic activity was determined at pH 4.0 in 0.1 mol/L Tris reagent, whereas the renal isoenzyme activity was determined at pH 5.6 in presence of 0.05 mol/L turanose reagent. N-Acetyl-beta-D-glucosaminidases A and B were determined by their different behaviors on heating. The corresponding reference intervals for each enzyme were calculated from results for 40 controls: acid alpha-glucosidase (0.024 +/- 0.010 U/L), renal alpha-glucosidase (0.035 +/- 0.012 U/L), N-acetyl-beta-D- glucosaminidase A (10.2 +/- 2.9 U/L), and N-acetyl-beta-D- glucosaminidase B (4.4 +/- 2.1 U/L).


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 Clin. Chem.Home page
L. F. Perez and J. C. Tutor
Assay of ß-N-acetylhexosaminidase isoenzymes in different biological specimens by means of determination of their activation energies
Clin. Chem., February 1, 1998; 44(2): 226 - 231.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Association for Clinical Chemistry.