Clinical Chemistry
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Clinical Chemistry 38: 292-298, 1992;
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Clinical Chemistry, Vol 38, 292-298, Copyright © 1992 by American Association for Clinical Chemistry

Identification of urinary calculi by Raman laser fiber optics spectroscopy

TD Hong, D Phat, P Plaza, M Daudon and NQ Dao
Laboratoire PCM, U.R.A. D0441 du CNRS, Ecole Centrale de Paris, Chatenay-Malabry, France.

Human calculi of various compositions were automatically identified by using near-infrared excitation Fourier-transform Raman spectrometry. After having built a 150-compound Raman library as a first step, we used a commercial software for infrared spectra (program BIRSY, from Bruker) to determine the composition of different calculi. Good results were obtained for both classical Raman laser and Raman laser fiber optics spectroscopies. With the use of a natural biological medium, e.g., urine, to mimic as closely as possible clinical in vivo conditions, the automatic search correctly identified the calculus composition with relatively good test quality; in some mixtures, however, the results can only be considered semi-quantitative at present, even after smoothing of the spectra.




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Copyright © 1992 by the American Association for Clinical Chemistry.