Clinical Chemistry, Vol 38, 381-385, Copyright © 1992 by American Association for Clinical Chemistry

Determination of serum glucose by isotope dilution mass spectrometry: candidate definitive method

F Magni, R Paroni, PA Bonini and MG Kienle
Laboratorio Centrale Analisi, Ospedale S. Raffaele, Milan, Italy.

We report a rather simple method to determine glucose concentration in serum, using isotope dilution mass spectrometry and [13C6]glucose as internal standard. The procedure involves a single step of sample purification and the conversion of the analyte into its aldononitrile pentaacetate. The between-day and within-day contribution to total variance for a single measurement was determined by assaying Standard Reference Material (SRM) 909 serum. The method was then applied to measurement of glucose concentration in three lyophilized sera: SRM 909 and two other commercially available sera. In the two studies, the concentration of SRM 909 serum was found to be 0.8% above and 0.3% below the reported value (6.25 mmol/L), respectively; the overall coefficient of variation for determinations in all sera ranged from 0.37% to 0.56%. The precision and the accuracy of the method satisfy the requirements for a Definitive Method.

The following articles in journals at HighWire Press have cited this article:

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