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Clinical Chemistry, Vol 38, 550-553, Copyright © 1992 by American Association for Clinical Chemistry
WY Craig, SE Poulin, NR Forster, LM Neveux, NJ Wald and TB Ledue
Foundation for Blood Research, Scarborough, ME 04070-0190.
Lipoprotein(a) [Lp(a)] was measured by both a radial immunodiffusion (RID) kit from Immuno AG (Zurich, Switzerland) and a Tint Elize enzyme- linked immunosorbent assay (ELISA) kit from CytRx Biopool Ltd. (Umea, Sweden) in serum samples that had been stored at -20 and -70 degrees C for six months. Storage temperature had no significant effect on the Lp(a) concentrations obtained by either method. After six months, mean Lp(a) degradation was 46% (95% confidence interval, 34-58%) with the RID kit; the ELISA data could not be compared between time points. In fresh sera, Lp(a) concentrations obtained by RID were 41% higher than by ELISA (because of differences in assay calibration materials), but in paired measurements of a set of 215 samples stored at -40 degrees C for an average of 10 years, Lp(a) concentrations were 62% lower by RID. This suggests that RID is more sensitive to the effects of long-term storage than is ELISA.
The following articles in journals at HighWire Press have cited this article:
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  J. M. Simo, J. Camps, E. Vilella, F. Gomez, A. Paul, and J. Joven Instability of Lipoprotein(a) in Plasma Stored at -70 {degrees}C : Effects of Concentration, Apolipoprotein(a) Genotype, and Donor Cardiovascular Disease Clin. Chem., September 1, 2001; 47(9): 1673 - 1678. [Abstract] [Full Text] [PDF]
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 U. Querfeld, S. Döpper, A. Gradehand, P. Kiencke, F. Wahn, and H.-J. Zeisel Long-Term Treatment with Growth Hormone Has No Persisting Effect on Lipoprotein(a) in Patients with Turner's Syndrome J. Clin. Endocrinol. Metab., March 1, 1999; 84(3): 967 - 970. [Abstract] [Full Text]
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W. Y. Craig, L. M. Neveux, G. E. Palomaki, M. M. Cleveland, and J. E. Haddow Lipoprotein(a) as a risk factor for ischemic heart disease: metaanalysis of prospective studies Clin. Chem., November 1, 1998; 44(11): 2301 - 2306. [Abstract] [Full Text] [PDF]
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 J. H. Stein and R. S. Rosenson Lipoprotein Lp(a) Excess and Coronary Heart Disease Arch Intern Med, June 9, 1997; 157(11): 1170 - 1176. [Abstract] [PDF]
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 F. Kronenberg, E. Trenkwalder, H. Dieplinger, and G. Utermann Lipoprotein(a) in Stored Plasma Samples and the Ravages of Time: Why Epidemiological Studies Might Fail Arterioscler Thromb Vasc Biol, December 1, 1996; 16(12): 1568 - 1572. [Abstract] [Full Text]
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 A. G. Bostom, L. A. Cupples, J. L. Jenner, J. M. Ordovas, L. J. Seman, P. W. F. Wilson, E. J. Schaefer, and W. P. Castelli Elevated Plasma Lipoprotein(a) and Coronary Heart Disease in Men Aged 55 Years and Younger: A Prospective Study JAMA, August 21, 1996; 276(7): 544 - 548. [Abstract] [PDF]
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 R. L. Desmarais, I. J. Sarembock, C. R. Ayers, S. M. Vernon, E. R. Powers, and L. W. Gimple Elevated Serum Lipoprotein(a) Is a Risk Factor for Clinical Recurrence After Coronary Balloon Angioplasty Circulation, March 1, 1995; 91(5): 1403 - 1409. [Abstract] [Full Text]
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E. J. Schaefer, S. Lamon-Fava, J. L. Jenner, J. R. McNamara, J. M. Ordovas, C. E. Davis, J. M. Abolafia, K. Lippel, and R. I. Levy Lipoprotein(a) Levels and Risk of Coronary Heart Disease in Men: The Lipid Research Clinics Coronary Primary Prevention Trial JAMA, April 6, 1994; 271(13): 999 - 1003. [Abstract] [PDF]
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