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Clinical Chemistry 38: 575-577, 1992;
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Clinical Chemistry, Vol 38, 575-577, Copyright © 1992 by American Association for Clinical Chemistry

Effect of in vitro hemolysis on 25 common biochemical tests

D Yucel and K Dalva
Clinical Biochemistry Laboratory, Yuksek Ihtisas Hastanesi (High Specialization Hospital), Ankara, Turkey.

Clinical chemists frequently encounter hemolyzed samples. Our study examines the effects of hemolysis on the results of 25 common biochemical tests. We collected 60 15-mL blood samples from inpatients and outpatients and mechanically hemolyzed 10 mL of the samples in a two-step procedure. We classified serum from these samples as being nonhemolyzed, moderately hemolyzed, or severely hemolyzed and then performed 25 common biochemical tests. Statistical analysis of the results showed that hemolysis had the greatest effect on the lactate dehydrogenase, acid phosphatase, and potassium tests.


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Copyright © 1992 by the American Association for Clinical Chemistry.