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Clinical Chemistry 38: 704-709, 1992;
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Clinical Chemistry, Vol 38, 704-709, Copyright © 1992 by American Association for Clinical Chemistry

Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid

MJ Richard, B Portal, J Meo, C Coudray, A Hadjian and A Favier
Laboratoire de Biochimie C, Centre Hospitalier Regional Universitaire A, Grenoble, France.

The determination of thiobarbituric acid reactants (TBARs) is a widely used method for investigating overall lipid peroxidation. An assay kit that could be used with plasma and lipid fractions would facilitate standardization of the method. The results reported here indicate that the malondialdehyde (MDA) kit manufactured by Sobioda (Grenoble, France) complies with criteria of good analytical practices. The detection limit was 0.11 mumol of MDA per liter of plasma. The within- run (CV = 1.8-3.3%) and between-run (CV = 3.3-4.4%) precisions were acceptable. The analytical recovery of MDA after supplementing human plasma samples with tetraethoxypropane standards varied from 88% to 100%. The mean (SD) lipoperoxide concentration determined in 32 healthy adults, ages 20-40 years, was 2.51 (0.25) mumol/L. No significant sex- related difference was noted: 2.57 (0.28) in men vs 2.44 (0.20) mumol/L in women. Applying the method to lipid fractions showed that lipoprotein fractions contain relatively little MDA: 0.07 (0.03) mumol/L of plasma for very-low-density lipoproteins and 0.19 (0.10) mumol/L for low-density lipoproteins.


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Copyright © 1992 by the American Association for Clinical Chemistry.