Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 38: 730-735, 1992;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bright, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bright, G. M.

Clinical Chemistry, Vol 38, 730-735, Copyright © 1992 by American Association for Clinical Chemistry

Quantification of islet cell antibodies by microscope photometry and antibody titration compared

GM Bright
Nemours Children's Clinic, Jacksonville, FL 32207.

Islet cell antibodies can predict eventual insulin-dependent diabetes mellitus. In the standard method used by the Immunology of Diabetes Workshop (IDW), predictive power depends on antibody titer as determined by testing multiple serum dilutions. Here I report use of a microscope-based photometry system (MPS) to test whether islet fluorescence intensities from undiluted sera can predict IDW results. MPS testing of 120 undiluted IDW specimens correctly identified 27 of 31 positive and 84 of 89 negative specimens. The relationship between IDW consensus values and MPS readings of undiluted sera is: IDW = 2.45 MPS - 1.44 (r = 0.67, P less than 0.001). Thus, corrected islet intensities from undiluted sera correlated well with IDW values. However, the ability of MPS to predict high IDW consensus values is limited, which may reflect the variable antigenic content of pancreas specimens; moreover, background correction as currently applied is incomplete; nonspecific immunofluorescence appears to emanate from within the islet.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by the American Association for Clinical Chemistry.