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Clinical Chemistry 38: 808-812, 1992;
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Clinical Chemistry, Vol 38, 808-812, Copyright © 1992 by American Association for Clinical Chemistry

Automated homogeneous liposome immunoassay systems for anticonvulsant drugs

K Kubotsu, S Goto, M Fujita, H Tuchiya, M Kida, S Takano, S Matsuura and I Sakurabayashi
Osaka Research Laboratory, Wako Pure Chemical Industries, Ltd., Japan.

We developed automated homogeneous immunoassays, based on immunolysis of liposomes, for measuring phenytoin, phenobarbital, and carbamazepine from serum. Liposome lysis was detected spectrophotometrically from entrapped glucose-6-phosphate dehydrogenase activity. The procedure was fully automated on a routine automated clinical analyzer. Within-run, between-run, dilution, and recovery tests showed good accuracies and reproducibilities. Bilirubin, hemoglobin, triglycerides, and Intrafat did not affect assay results. The results obtained by liposome immunoassays for phenytoin, phenobarbital, and carbamazepine correlated well with those obtained by enzyme-multiplied immunoassay (Syva EMIT) kits (r = 0.995, 0.986, and 0.988, respectively) and fluorescence polarization immunoassay (Abbott TDx) kits (r = 0.990, 0.991, and 0.975, respectively). The proposed method should be useful for monitoring anticonvulsant drug concentrations in blood.




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Copyright © 1992 by the American Association for Clinical Chemistry.