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Clinical Chemistry 38: 853-859, 1992;
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Clinical Chemistry, Vol 38, 853-859, Copyright © 1992 by American Association for Clinical Chemistry

Dissociation-enhanced lanthanide fluorescence immunoassay of lipoprotein(a) in serum

G Jurgens, A Hermann, D Aktuna and W Petek
Institute of Medical Biochemistry, Karl-Franzens Universitat Graz, Austria.

Lipoprotein(a), a human serum lipoprotein structurally related to low- density lipoprotein (LDL), contains in addition to apolipoprotein B (apo B) apolipoprotein(a) [apo(a)], a glycoprotein with a strong homology to plasminogen. Lp(a) is a risk factor for coronary heart disease and ischemic cerebrovascular disease. Several immunological techniques are used to quantify Lp(a) in human serum, including radioimmunoassays, rocket immunoelectrophoresis, and enzyme-linked immunosorbent assays. Only the last method is suitable for large-scale clinical studies. We describe another solid-phase immunoassay, based on the dissociation-enhanced lanthanide fluorescence system Delfia (Wallac Oy), and outline the technical details. A polyclonal antiserum directed against Lp(a) was used as the capture antibody. Two kinds of detection antibodies were applied, a polyclonal antiserum against apo B and the polyclonal antiserum against Lp(a). The results agree excellently with the values estimated by rocket immunoelectrophoresis. This assay is easily established, measures Lp(a) in a wide concentration range, and is suitable for screening large populations.


The following articles in journals at HighWire Press have cited this article:


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 Arterioscler. Thromb. Vasc. Bio.Home page
A. Hammer, G. Kager, G. Dohr, H. Rabl, I. Ghassempur, and G. Jurgens
Generation, Characterization, and Histochemical Application of Monoclonal Antibodies Selectively Recognizing Oxidatively Modified ApoB-Containing Serum Lipoproteins
Arterioscler. Thromb. Vasc. Biol., May 1, 1995; 15(5): 704 - 713.
[Abstract] [Full Text]


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Copyright © 1992 by the American Association for Clinical Chemistry.