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Clinical Chemistry 38: 1342-1345, 1992;
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Clinical Chemistry, Vol 38, 1342-1345, Copyright © 1992 by American Association for Clinical Chemistry

Measuring albumin and calcium in serum in a dual test with the Hitachi 704

NO Leary, A Pembroke and PF Duggan
Biochemistry Laboratory, Regional Hospital, Cork, Ireland.

We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin less than or equal to 5 g/L and Intralipid less than or equal to 4 g/L; calcium is unaffected by bilirubin less than or equal to 600 mumol/L.




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