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Clinical Chemistry 38: 1488-1490, 1992;
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Clinical Chemistry, Vol 38, 1488-1490, Copyright © 1992 by American Association for Clinical Chemistry

Hemoglobin A1c by HPLC with the Pharmacia Mono S HR 5/N cation-exchange column: influence of sample protein load on optimal chromatographic conditions

JC Philcox, MR Haywood and AM Rofe
Division of Clinical Chemistry, Institute of Medical and Veterinary Science, Adelaide, South Australia.

The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.


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Copyright © 1992 by the American Association for Clinical Chemistry.