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Clinical Chemistry 38: 1749-1751, 1992;
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Clinical Chemistry, Vol 38, 1749-1751, Copyright © 1992 by American Association for Clinical Chemistry

A simplified procedure for eliminating the negative interference of bilirubin in the Jaffe reaction for creatinine

N O'Leary, A Pembroke and PF Duggan
Biochemistry Laboratory, Regional Hospital, Cork, Ireland.

The negative interference by bilirubin in the measurement of creatinine by the Jaffe procedure is well documented. We describe a procedure for the elimination of interfering bilirubin by oxidation to biliverdin through the addition of potassium ferricyanide before the alkaline picrate reagent. We detected no negative interference with the addition of as much as 600 mumol/L bilirubin. The procedure shows good agreement with a recently described blank rate method for analyzing icteric samples.


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