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Clinical Chemistry 38: 1752-1755, 1992;
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Clinical Chemistry, Vol 38, 1752-1755, Copyright © 1992 by American Association for Clinical Chemistry

Rapid and sensitive method for erythropoietin determination in serum

M Tanebe, S Teshima, T Hanyu and Y Hayashi
Tsuruga Enzyme Plant, Toyobo Co., Ltd., Fukui, Japan.

An enzyme-linked immunosorbent assay (ELISA) method for erythropoietin determination has been established by using two kinds of monoclonal antibodies with specific affinities to erythropoietin. Besides being rapid (2.5 h) and sensitive (detection limit 0.3 int. unit/L), the present method gives accurate results and is easy to perform. The method may be clinically applicable for discriminative diagnosis of polycythemia and analyses of various anemic conditions.




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