Clinical Chemistry
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Clinical Chemistry 38: 1792-1795, 1992;
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Clinical Chemistry, Vol 38, 1792-1795, Copyright © 1992 by American Association for Clinical Chemistry

Simple method for quantifying alpha-tocopherol in low-density+very-low- density lipoproteins and in high-density lipoproteins

G Carcelain, F David, S Lepage, D Bonnefont-Rousselot, J Delattre, A Legrand, J Peynet and S Troupel
Laboratoire de Biochimie, Hopital de la Salpetriere, Paris, France.

We assessed the distribution of alpha-tocopherol in serum lipoprotein samples after separating the lipoprotein fractions by either sequential ultracentrifugation or selective precipitation with sodium phosphotungstate-magnesium chloride reagent. alpha-Tocopherol concentrations were determined by reversed-phase high-performance liquid chromatography. After ultracentrifugation, we found that in men, low- and very-low-density serum lipoproteins (LDL-VLDL) contained 53.6% of alpha-tocopherol vs 46.4% in high-density lipoproteins (HDL). In women, serum LDL-VLDL contained 45.6% alpha-tocopherol after ultracentrifugation vs 54.4% in HDL. After selective precipitation, the proportions of alpha-tocopherol in men were 56.1% in LDL-VLDL vs 43.9% in HDL, and in women, 45.4% in LDL-VLDL vs 54.6% in HDL. After selective precipitation, alpha-tocopherol recovery from whole lipoprotein fractions was 97% to 100% vs 80% after ultracentrifugation, thus allowing more accurate alpha-tocopherol quantification than after separation by ultracentrifugation.


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 J. Nutr.Home page
E. Lanza, M. R. Forman, E. J. Johnson, R. A. Muesing, B. I. Graubard, and G. R. Beecher
alpha -Tocopherol Concentrations in Plasma but not in Lipoproteins Fluctuate during the Menstrual Cycle in Healthy Premenopausal Women
J. Nutr., July 1, 1998; 128(7): 1150 - 1155.
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Copyright © 1992 by the American Association for Clinical Chemistry.