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Clinical Chemistry 39: 129-131, 1993;
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Clinical Chemistry, Vol 39, 129-131, Copyright © 1993 by American Association for Clinical Chemistry

Phenylalanine determined in plasma with use of phenylalanine dehydrogenase and a centrifugal analyzer

MA Vilaseca, C Farre and F Ramon
Servei de Bioquimica, Hospital Infantil Sant Joan de Deu, Barcelona, Spain.

Quantitative determination of plasma phenylalanine (Phe) is essential for the diagnosis of phenylketonuria (PKU) and the control of dietary therapy of PKU patients. We have adapted a spectrophotometric method (Clin Chim Acta 1991;201:95-8) based on phenylalanine dehydrogenase (EC 1.4.1.-) for rapid and accurate determinations of Phe with the Cobas Fara II centrifugal analyzer. The method is based on the NAD(H)- dependent reductive deamination of Phe with large amounts of phenylalanine dehydrogenase, which catalyzes the formation of phenylpyruvate at pH 10.8. Combining the specificity of the phenylalanine dehydrogenase with the precision, accuracy, and considerable time-saving of an automated system is useful for monitoring PKU patients in a children's hospital.




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Copyright © 1993 by the American Association for Clinical Chemistry.