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Clinical Chemistry 39: 134-138, 1993;
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Clinical Chemistry, Vol 39, 134-138, Copyright © 1993 by American Association for Clinical Chemistry

Plasma and urinary oxalate and glycolate in healthy subjects

L Hagen, VR Walker and RA Sutton
Department of Medicine, University of British Columbia, Vancouver, Canada.

High-performance ion chromatography (HPIC) has been widely used for oxalate analysis and, more recently, for glycolate analysis. We describe a procedure for sample preparation in which the plasma ultrafiltrate is acidified during harvesting with a cation-exchange resin, and the chloride is removed before the ion chromatography, which is performed with a newly developed AS10 column. The same ultrafiltrate sample is analyzed for glycolate. For plasma oxalate, the mean recovery of sample in eluted fractions was 95-96%, and intraassay CV was 6.2- 8.1%. The reference interval (mean +/- 2 SD) for men was 0.8-3.2 mumol/L and for women, 1.0-2.6 mumol/L. For urinary oxalate, the reference interval for men was 175-560 mumol/day and for women, 107-432 mumol/day. For plasma glycolate, the mean analytical recovery was 96- 98%, and the intra-assay CV was 2.4-6.2%. The reference interval for men was 1.9-7.5 mumol/L and for women, 1.4-7.4 mumol/L. For urinary glycolate, the reference interval for men was 0-1400 mumol/day and for women, 91-1001 mumol/day.


The following articles in journals at HighWire Press have cited this article:


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 Am. J. Physiol. Cell Physiol.Home page
P. R. S. Baker, S. D. Cramer, M. Kennedy, D. G. Assimos, and R. P. Holmes
Glycolate and glyoxylate metabolism in HepG2 cells
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1359 - C1365.
[Abstract] [Full Text] [PDF]


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Copyright © 1993 by the American Association for Clinical Chemistry.