Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 97-103, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lenders, J. W.
Right arrow Articles by Kopin, I. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lenders, J. W.
Right arrow Articles by Kopin, I. J.

Clinical Chemistry, Vol 39, 97-103, Copyright © 1993 by American Association for Clinical Chemistry

Determination of metanephrines in plasma by liquid chromatography with electrochemical detection

JW Lenders, G Eisenhofer, I Armando, HR Keiser, DS Goldstein and IJ Kopin
Clinical Neuroscience Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

Metanephrines are O-methylated metabolites of catecholamines. We report the use of liquid chromatography with electrochemical detection to determine plasma concentrations of normetanephrine (NMN) and metanephrine (MN). Plasma NMN and MN in 32 normal volunteers and inpatients were compared with concentrations in 23 patients with pheochromocytoma. Metanephrines were adsorbed from plasma onto a cation- exchange column and eluted with ammoniacal methanol. The dried residue was dissolved in mobile phase and injected onto a reversed-phase column. Recoveries of NMN and MN from 1 mL of plasma averaged 50-70%, and results varied linearly with quantity injected over a range of 0.13- 55 pmol. The detection limit was 25 fmol for NMN and 50 fmol for MN. Intra-assay CVs were < 5%. In normal volunteers and inpatients, plasma concentrations of NMN ranged between 0.12 and 0.73 nmol/L (mean 0.38 nmol/L), and MN between 0.06 and 0.63 nmol/L (mean 0.19 nmol/L). Plasma NMN concentrations were increased in all 23 patients with pheochromocytoma (range 1-172 nmol/L), whereas MN concentrations (range 0.10-382 nmol/L) were increased in only 9 patients. The assay method is reliable and sensitive and offers an approach to examine the extraneuronal metabolism of catecholamines. The method may also be useful in the diagnosis of pheochromocytoma.


The following articles in journals at HighWire Press have cited this article:


Home page
 Endocr Relat CancerHome page
T. Zelinka, H. J L M Timmers, A. Kozupa, C. C Chen, J. A Carrasquillo, J. C Reynolds, A. Ling, G. Eisenhofer, I. Lazurova, K. T Adams, et al.
Role of positron emission tomography and bone scintigraphy in the evaluation of bone involvement in metastatic pheochromocytoma and paraganglioma: specific implications for succinate dehydrogenase enzyme subunit B gene mutations
Endocr. Relat. Cancer, March 1, 2008; 15(1): 311 - 323.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
A. Karagiannis, D. P Mikhailidis, V. G Athyros, and F. Harsoulis
Pheochromocytoma: an update on genetics and management
Endocr. Relat. Cancer, December 1, 2007; 14(4): 935 - 956.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
W. H.A. de Jong, K. S. Graham, J. C. van der Molen, T. P. Links, M. R. Morris, H. A. Ross, E. G.E. de Vries, and I. P. Kema
Plasma Free Metanephrine Measurement Using Automated Online Solid-Phase Extraction HPLC Tandem Mass Spectrometry
Clin. Chem., September 1, 2007; 53(9): 1684 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
V. B. Wyller, K. Godang, L. Morkrid, J. P. Saul, E. Thaulow, and L. Walloe
Abnormal Thermoregulatory Responses in Adolescents With Chronic Fatigue Syndrome: Relation to Clinical Symptoms
Pediatrics, July 1, 2007; 120(1): e129 - e137.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. S. Landa, S. I. Garcia, M. L. Schuman, A. Burgueno, A. L. Alvarez, F. E. Saravia, C. Gemma, and C. J. Pirola
Knocking down the diencephalic thyrotropin-releasing hormone precursor gene normalizes obesity-induced hypertension in the rat
Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1388 - E1394.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
H. J. L. M. Timmers, A. Kozupa, G. Eisenhofer, M. Raygada, K. T. Adams, D. Solis, J. W. M. Lenders, and K. Pacak
Clinical Presentations, Biochemical Phenotypes, and Genotype-Phenotype Correlations in Patients with Succinate Dehydrogenase Subunit B-Associated Pheochromocytomas and Paragangliomas
J. Clin. Endocrinol. Metab., March 1, 2007; 92(3): 779 - 786.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
F. M. Brouwers, G. Eisenhofer, J. J. Tao, J. A. Kant, K. T. Adams, W. M. Linehan, and K. Pacak
High Frequency of SDHB Germline Mutations in Patients with Malignant Catecholamine-Producing Paragangliomas: Implications for Genetic Testing
J. Clin. Endocrinol. Metab., November 1, 2006; 91(11): 4505 - 4509.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
T.-T. Huynh, K. Pacak, F. M Brouwers, M. S Abu-Asab, R. A Worrell, M. M Walther, A. G Elkahloun, D. S Goldstein, S. Cleary, and G. Eisenhofer
Different expression of catecholamine transporters in phaeochromocytomas from patients with von Hippel-Lindau syndrome and multiple endocrine neoplasia type 2
Eur. J. Endocrinol., October 1, 2005; 153(4): 551 - 563.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Eisenhofer, D. S. Goldstein, P. Sullivan, G. Csako, F. M. Brouwers, E. W. Lai, K. T. Adams, and K. Pacak
Biochemical and Clinical Manifestations of Dopamine-Producing Paragangliomas: Utility of Plasma Methoxytyramine
J. Clin. Endocrinol. Metab., April 1, 2005; 90(4): 2068 - 2075.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. Eisenhofer, J. W.M. Lenders, D. S. Goldstein, M. Mannelli, G. Csako, M. M. Walther, F. M. Brouwers, and K. Pacak
Pheochromocytoma Catecholamine Phenotypes and Prediction of Tumor Size and Location by Use of Plasma Free Metanephrines
Clin. Chem., April 1, 2005; 51(4): 735 - 744.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. C. Heider, B. G. Davis, and E. L. Frank
Nonparametric Determination of Reference Intervals for Plasma Metanephrine and Normetanephrine
Clin. Chem., December 1, 2004; 50(12): 2381 - 2384.
[Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
G. Eisenhofer, I. J. Kopin, and D. S. Goldstein
Catecholamine Metabolism: A Contemporary View with Implications for Physiology and Medicine
Pharmacol. Rev., September 1, 2004; 56(3): 331 - 349.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. M. Sawka, A. Gafni, L. Thabane, and W. F. Young Jr.
The Economic Implications of Three Biochemical Screening Algorithms for Pheochromocytoma
J. Clin. Endocrinol. Metab., June 1, 2004; 89(6): 2859 - 2866.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
B. E. de Galan, C. J. Tack, J. J. Willemsen, C. G. J. Sweep, P. Smits, and J. W. M. Lenders
Plasma Metanephrine Levels Are Decreased in Type 1 Diabetic Patients with a Severely Impaired Epinephrine Response to Hypoglycemia, Indicating Reduced Adrenomedullary Stores of Epinephrine
J. Clin. Endocrinol. Metab., May 1, 2004; 89(5): 2057 - 2061.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. A. Lagerstedt, D. J. O'Kane, and R. J. Singh
Measurement of Plasma Free Metanephrine and Normetanephrine by Liquid Chromatography-Tandem Mass Spectrometry for Diagnosis of Pheochromocytoma
Clin. Chem., March 1, 2004; 50(3): 603 - 611.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. J. Willemsen, C.G.J. Sweep, J. W.M. Lenders, and H. A. Ross
Stability of Plasma Free Metanephrines during Collection and Storage as Assessed by an Optimized HPLC Method with Electrochemical Detection
Clin. Chem., November 1, 2003; 49(11): 1951 - 1953.
[Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
Y. C. Kudva, A. M. Sawka, and W. F. Young Jr.
The Laboratory Diagnosis of Adrenal Pheochromocytoma: The Mayo Clinic Experience
J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4533 - 4539.
[Full Text] [PDF]

Home page
 Endocr. Rev.Home page
E. L. Bravo and R. Tagle
Pheochromocytoma: State-of-the-Art and Future Prospects
Endocr. Rev., August 1, 2003; 24(4): 539 - 553.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Eisenhofer, D. S. Goldstein, M. M. Walther, P. Friberg, J. W. M. Lenders, H. R. Keiser, and K. Pacak
Biochemical Diagnosis of Pheochromocytoma: How to Distinguish True- from False-Positive Test Results
J. Clin. Endocrinol. Metab., June 1, 2003; 88(6): 2656 - 2666.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
D. S. Goldstein, C. Holmes, Y. Sharabi, S. Brentzel, and G. Eisenhofer
Plasma levels of catechols and metanephrines in neurogenic orthostatic hypotension
Neurology, April 22, 2003; 60(8): 1327 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. M. Sawka, R. Jaeschke, R. J. Singh, and W. F. Young Jr.
A Comparison of Biochemical Tests for Pheochromocytoma: Measurement of Fractionated Plasma Metanephrines Compared with the Combination of 24-Hour Urinary Metanephrines and Catecholamines
J. Clin. Endocrinol. Metab., February 1, 2003; 88(2): 553 - 558.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Weise, G. Eisenhofer, and D. P. Merke
Pubertal and Gender-Related Changes in the Sympathoadrenal System in Healthy Children
J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5038 - 5043.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
E. Charmandari, G. Eisenhofer, S. L. Mehlinger, A. Carlson, R. Wesley, M. F. Keil, G. P. Chrousos, M. I. New, and D. P. Merke
Adrenomedullary Function May Predict Phenotype and Genotype in Classic 21-Hydroxylase Deficiency
J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3031 - 3037.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Weise, D. P. Merke, K. Pacak, M. M. Walther, and G. Eisenhofer
Utility of Plasma Free Metanephrines for Detecting Childhood Pheochromocytoma
J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 1955 - 1960.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
E. Charmandari, M. Weise, S. R. Bornstein, G. Eisenhofer, M. F. Keil, G. P. Chrousos, and D. P. Merke
Children with Classic Congenital Adrenal Hyperplasia Have Elevated Serum Leptin Concentrations and Insulin Resistance: Potential Clinical Implications
J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2114 - 2120.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
J. W. M. Lenders, K. Pacak, M. M. Walther, W. M. Linehan, M. Mannelli, P. Friberg, H. R. Keiser, D. S. Goldstein, and G. Eisenhofer
Biochemical Diagnosis of Pheochromocytoma: Which Test Is Best?
JAMA, March 20, 2002; 287(11): 1427 - 1434.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. L. Taylor and R. J. Singh
Validation of Liquid Chromatography-Tandem Mass Spectrometry Method for Analysis of Urinary Conjugated Metanephrine and Normetanephrine for Screening of Pheochromocytoma
Clin. Chem., March 1, 2002; 48(3): 533 - 539.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
K. Pacak, D. S. Goldstein, J. L. Doppman, B. L. Shulkin, R. Udelsman, and G. Eisenhofer
A ""Pheo"" Lurks: Novel Approaches for Locating Occult Pheochromocytoma
J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3641 - 3646.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Roden, W. Raffesberg, W. Raber, E. Bernroider, B. Niederle, W. Waldhausl, and S. Gasic
Quantification of Unconjugated Metanephrines in Human Plasma without Interference by Acetaminophen
Clin. Chem., June 1, 2001; 47(6): 1061 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Eisenhofer, M. M. Walther, T.-T. Huynh, S.-T. Li, S. R. Bornstein, A. Vortmeyer, M. Mannelli, D. S. Goldstein, W. M. Linehan, J. W. M. Lenders, et al.
Pheochromocytomas in von Hippel-Lindau Syndrome and Multiple Endocrine Neoplasia Type 2 Display Distinct Biochemical and Clinical Phenotypes
J. Clin. Endocrinol. Metab., May 1, 2001; 86(5): 1999 - 2008.
[Abstract] [Full Text]

Home page
 HypertensionHome page
F. Rao, H. R. Keiser, and D. T. O'Connor
Malignant Pheochromocytoma : Chromaffin Granule Transmitters and Response to Treatment
Hypertension, December 1, 2000; 36(6): 1045 - 1052.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
D. P. Merke, G. P. Chrousos, G. Eisenhofer, M. Weise, M. F. Keil, A. D. Rogol, J. J. V. Wyk, and S. R. Bornstein
Adrenomedullary Dysplasia and Hypofunction in Patients with Classic 21-Hydroxylase Deficiency
N. Engl. J. Med., November 9, 2000; 343(19): 1362 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
W. Raber, W. Raffesberg, M. Bischof, C. Scheuba, B. Niederle, S. Gasic, W. Waldhausl, and M. Roden
Diagnostic Efficacy of Unconjugated Plasma Metanephrines for the Detection of Pheochromocytoma
Arch Intern Med, October 23, 2000; 160(19): 2957 - 2963.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
G. Eisenhofer, J. W.M. Lenders, W. M. Linehan, M. M. Walther, D. S. Goldstein, and H. R. Keiser
Plasma Normetanephrine and Metanephrine for Detecting Pheochromocytoma in von Hippel-Lindau Disease and Multiple Endocrine Neoplasia Type 2
N. Engl. J. Med., June 17, 1999; 340(24): 1872 - 1879.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
I. J. Kopin, B. Rundqvist, P. Friberg, J. Lenders, D. S. Goldstein, and G. Eisenhofer
Different relationships of spillover to release of norepinephrine in human heart, kidneys, and forearm
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1998; 275(1): R165 - R173.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Eisenhofer, H. Keiser, P. Friberg, E. Mezey, T.-T. Huynh, B. Hiremagalur, T. Ellingson, S. Duddempudi, A. Eijsbouts, and J. W. M. Lenders
Plasma Metanephrines Are Markers of Pheochromocytoma Produced by Catechol-O-Methyltransferase Within Tumors
J. Clin. Endocrinol. Metab., June 1, 1998; 83(6): 2175 - 2185.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. Eisenhofer, B. Rundqvist, and P. Friberg
Determinants of cardiac tyrosine hydroxylase activity during exercise-induced sympathetic activation in humans
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 1998; 274(3): R626 - R634.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Eisenhofer, A. Aneman, P. Friberg, D. Hooper, L. Fandriks, H. Lonroth, B. Hunyady, and E. Mezey
Substantial Production of Dopamine in the Human Gastrointestinal Tract
J. Clin. Endocrinol. Metab., November 1, 1997; 82(11): 3864 - 3871.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
G. Eisenhofer, P. Friberg, B. Rundqvist, A. A. Quyyumi, G. Lambert, D. M. Kaye, I. J. Kopin, D. S. Goldstein, and M. D. Esler
Cardiac Sympathetic Nerve Function in Congestive Heart Failure
Circulation, May 1, 1996; 93(9): 1667 - 1676.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.