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Clinical Chemistry 39: 2090-2097, 1993;
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Clinical Chemistry, Vol 39, 2090-2097, Copyright © 1993 by American Association for Clinical Chemistry

Fully automated assay of glycohemoglobin with the Abbott IMx analyzer: novel approaches for separation and detection

DH Wilson, JP Bogacz, CM Forsythe, PJ Turk, TL Lane, RC Gates and DR Brandt
Department of Thyroid, Metabolic, and Cardiovascular Diagnostics, Abbott Laboratories, Abbott Park, IL 60064.

We describe a novel assay for measuring glycohemoglobin directly from anticoagulated whole blood with the Abbott IMx analyzer. The glycohemoglobin is labeled with a soluble polyanionic affinity reagent and the anionic complex is then captured with a cationic solid-phase matrix. Glycohemoglobin is quantified by measuring the quenching by heme of the static fluorescence from an added fluorophore. The assay is standardized to report both percent total glycohemoglobin (%GHb) and percent hemoglobin A1c (%HbA1c). Glucose, bilirubin, triglycerides, labile fraction, and hemoglobin variants do not interfere in the assay. Within- and between-run CVs are approximately 4-5%, with total CVs of approximately 6.5%. Highly significant linear correlations (r > 0.97) were obtained in comparison studies with two major assay methodologies. The time to obtain one result is approximately 10 min (including assay of a control), 56 min for 22 results. We describe the development, standardization, and validation of this new method.


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