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Clinical Chemistry 39: 2150-2154, 1993;
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Clinical Chemistry, Vol 39, 2150-2154, Copyright © 1993 by American Association for Clinical Chemistry

Modified assay of prostate-specific antigen with a detection limit < 0.01 microgram/L

RJ Liedtke, G Kroon and JD Batjer
Laboratory of Pathology of Seattle, Inc., WA 98104.

We modified the Hybritech Tandem-E prostate-specific antigen (PSA) assay by increasing the sample volume, increasing enzyme-substrate incubation time, and using diethanolamine buffer. Our modified method has a detection limit of 0.009 microgram/L (P < 0.01). The assay curve is linear from 0.01 to 1.0 micrograms/L and has an overall assay time of about 4 h. Linear plots are obtained when the 1.0 micrograms/L standard is diluted with either matrix buffer or serum from men containing PSA < 0.01 microgram/L. Recovery of PSA (0.10 microgram/L) added to serum from men averaged 94%. Interassay CVs were 13%, 7%, and 4% at PSA concentrations of 0.04, 0.07, and 0.30 micrograms/L, respectively (n = 33). This assay should be useful in the detection of early recurrence of prostate cancer after radical prostatectomy.


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S.-F. Chen, Y. Xu, and M. P.-C. Ip
Electrochemical Enzyme Immunoassay for Serum Prostate-Specific Antigen at Low Concentrations
Clin. Chem., August 1, 1997; 43(8): 1459 - 1461.
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