Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 2157-2162, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gunneberg, A.
Right arrow Articles by Kalsheker, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gunneberg, A.
Right arrow Articles by Kalsheker, N.

Clinical Chemistry, Vol 39, 2157-2162, Copyright © 1993 by American Association for Clinical Chemistry

Competitive assay to improve the specificity of detection of single- point mutations in alpha 1-antitrypsin deficiency

A Gunneberg, G Scobie, K Hayes and N Kalsheker
Department of Clinical Chemistry, Southmead Hospital, Westbury-on-Trym, Bristol, UK.

Allele-specific oligonucleotides are used widely for the detection of single point mutations in genes. A modification of this assay based on competition has been developed for detection of the Z mutation of alpha 1-antitrypsin (alpha 1-AT). The normal alpha 1-AT allele is referred to as M, and the Z mutation arises from a single base substitution. Amplified DNA products corresponding to homozygous M, heterozygous MZ, and homozygous Z obtained by the polymerase chain reaction were incubated with a twofold molar excess of unlabeled oligonucleotide prior to hybridization with a radiolabeled oligonucleotide. Thus, initial incubation with unlabeled M-specific oligonucleotide was followed by hybridization with radiolabeled Z-specific oligonucleotide, and vice versa. This assay increased the specificity of single-point mutation detection three- to four-fold. Furthermore, specific hybridization was obtained at a lower temperature as a consequence of improving the signal-to-noise ratio.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
L. Prix, P. Uciechowski, B. Bockmann, M. Giesing, and A. J. Schuetz
Diagnostic Biochip Array for Fast and Sensitive Detection of K-ras Mutations in Stool
Clin. Chem., March 1, 2002; 48(3): 428 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. H.W. Klaassen, M. de Metz, Y. van Aarssen, and J. Janssen
{{alpha}}1-Antitrypsin Deficiency as a Result of Compound Heterozygosity for the Z and MHeerlen Alleles
Clin. Chem., May 1, 2001; 47(5): 978 - 979.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.