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Clinical Chemistry 39: 2503-2508, 1993;
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Clinical Chemistry, Vol 39, 2503-2508, Copyright © 1993 by American Association for Clinical Chemistry

Optimal collection and storage conditions for catecholamine measurements in human plasma and urine

F Boomsma, G Alberts, L van Eijk, AJ Man in 't Veld and MA Schalekamp
Cardiovascular Research Institute COEUR, University Hospital Dijkzigt/Erasmus University, Rotterdam, The Netherlands.

Improvements in methodologies for measuring concentrations of catecholamines (CA) have led to an increasing use of these compounds as markers in the screening of patients and in long-term clinical trials. Because of the associated logistical problems, we have investigated the unresolved question of optimal conditions for sample preparation and for storage of plasma and urine samples. Results show that blood should be centrifuged within 1 h after collection; the use of a refrigerated centrifuge is not necessary. Once plasma is prepared, CA are stable for 1 day at 20 degrees C, 2 days at 4 degrees C, 1 month at -20 degrees C (or 6 months with added glutathione), and up to 1 year at -70 degrees C. CA are stable at 4 degrees C for 1 month in unpreserved urine and for 4 months in urine preserved with EDTA and sodium metabisulfite. In acidified urine, CA were nearly unchanged after 1 year at 4 and -20 degrees C.


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Copyright © 1993 by the American Association for Clinical Chemistry.