Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 251-256, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schilling, K.
Right arrow Articles by Horn, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schilling, K.
Right arrow Articles by Horn, A.

Clinical Chemistry, Vol 39, 251-256, Copyright © 1993 by American Association for Clinical Chemistry

Multiwavelength photometry of thermochromic indicator solutions for temperature determination in multicuvettes

K Schilling, GA Cumme, E Hoffmann-Blume, H Hoppe and A Horn
Friedrich-Schiller-Universitat, Medizinische Fakultat, Institut fur Biochemie, Jena, Germany.

If buffer/indicator systems are used as optical thermometers in multicuvettes, the temperature resolution is limited by errors of optical measurements produced mainly by variations of pathlength and blank transmittance of the wells. Theoretical and practical approaches show that, in multicuvettes, a between-well temperature resolution of < 0.05 degrees C can be achieved by multiwavelength photometry with use of the Tris/cresol red indicator system. Using up to three absorbances (A0, A1, A2) measured in the same well at different wavelengths for calculation of differences, quotients, and quotients of differences, we found the optimum temperature signal to be In(A1/A2), with equal- ranking absorbances changing with temperature in opposite directions. We have used the method successfully to measure the temperature profiles and temporal dynamics of temperatures at all positions in two types of multicuvettes under controlled conditions, or not controlled thermostatically.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.