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Clinical Chemistry 39: 309-312, 1993;
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Clinical Chemistry, Vol 39, 309-312, Copyright © 1993 by American Association for Clinical Chemistry

Time-resolved immunofluorometric assay of complement C3: application to cerebrospinal fluid

O Gaillard, D Meillet, MC Diemert, L Musset, J Delattre, E Schuller and J Galli
Laboratoire d'Immunochimie, Hopital de la Salpetriere, Paris, France.

Complement components have a role in various neurological disorders. Complement C3 can be measured by immunochemical methods, but only radioimmunoassays and electroimmunodiffusion assays (EIDs) are sufficiently sensitive to be applied to biological fluids in which the C3 concentration is low, especially cerebrospinal fluid (CSF). We report a sandwich-type time-resolved immunofluorometric assay (TR-IFMA) for C3 in CSF. The linearity (0.7-3650 micrograms/L) and intra- (CV < 4.8%) and inter-assay (CV < 10.9%) precision were satisfactory and the results agreed with those of EID. The assay is extremely sensitive (< 1 microgram/L) and its analytical range is large and well suited to clinical applications. This simple TR-IFMA is thus a nonisotopic alternative to radioimmunoassay for the quantification of complement C3 in CSF.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
Z. Jiang, W. Huang, J. Li, M. Li, A. Liang, S. Zhang, and B. Chen
Nanogold Catalysis Based Immunoresonance-Scattering Spectral Assay for Trace Complement Component 3
Clin. Chem., January 1, 2008; 54(1): 116 - 123.
[Abstract] [Full Text] [PDF]


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Copyright © 1993 by the American Association for Clinical Chemistry.