Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 317-324, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simpson, E. R.
Right arrow Articles by Mendelson, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simpson, E. R.
Right arrow Articles by Mendelson, C. R.

Clinical Chemistry, Vol 39, 317-324, Copyright © 1993 by American Association for Clinical Chemistry

Tissue-specific promoters regulate aromatase cytochrome P450 expression

ER Simpson, MS Mahendroo, GD Means, MW Kilgore, CJ Corbin and CR Mendelson
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235-9051.

In humans, estrogen biosynthesis occurs in several tissue sites, including ovary, placenta, adipose, and brain. Recent work from our laboratory indicates that tissue-specific expression of aromatase cytochrome P450 (P450arom), the enzyme responsible for estrogen biosynthesis, is determined, in part, by the use of tissue-specific promoters. Thus, the expression of P450arom in human ovary appears to utilize a promoter proximal to the translation start site. This promoter is not utilized in placenta; instead, the promoter used to drive aromatase expression in placenta is > or = 40 kb upstream from the translational start site. In addition, a minor promoter used in the expression of a small proportion of placental transcripts is 9 kb upstream from the start of translation. Transcripts from these promoters are also expressed in other fetal tissues, including placenta- related cells such as JEG-3 choriocarcinoma cells and hydatidiform moles and other fetal tissues such as fetal liver. In adipose tissue, on the other hand, expression of P450arom may be achieved by yet another, adipose-specific promoter. The various 5'-untranslated exons unique for expression driven by each of these promoters are spliced into a common intron/exon boundary upstream from the translational start site. This means that the protein expressed in each of the various tissue-specific sites of estrogen biosynthesis is identical.


The following articles in journals at HighWire Press have cited this article:


Home page
 Toxicol SciHome page
J. T. Sanderson, J. Hordijk, M. S. Denison, M. F. Springsteel, M. H. Nantz, and M. van den Berg
Induction and Inhibition of Aromatase (CYP19) Activity by Natural and Synthetic Flavonoid Compounds in H295R Human Adrenocortical Carcinoma Cells
Toxicol. Sci., November 1, 2004; 82(1): 70 - 79.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. M. Komar and T. E. Curry Jr
Inverse Relationship Between the Expression of Messenger Ribonucleic Acid for Peroxisome Proliferator-Activated Receptor {gamma} and P450 Side Chain Cleavage in the Rat Ovary
Biol Reprod, August 1, 2003; 69(2): 549 - 555.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S. Kagabu, H. Kodama, J. Fukuda, A. Karube, M. Murata, and T. Tanaka
Inhibitory effects of nitric oxide on the expression and activity of aromatase in human granulosa cells
Mol. Hum. Reprod., May 1, 1999; 5(5): 396 - 401.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Li, R. M. Booze, and L. B. Hersh
Tissue-specific Expression of Rat Neutral Endopeptidase (Neprilysin) mRNAs
J. Biol. Chem., March 17, 1995; 270(11): 5723 - 5728.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.