Clinical Chemistry
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Clinical Chemistry 39: 472-476, 1993;
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Clinical Chemistry, Vol 39, 472-476, Copyright © 1993 by American Association for Clinical Chemistry

Monoclonal antibody-based immunoenzymometric assays of retinol-binding protein

AB Pereira, SK Nishida, JG Vieira, MT Lombardi, MS Silva, H Ajzen and OL Ramos
Laboratorio Fleury, Escola Paulista de Medicina, Sao Paulo, Brazil.

Retinol-binding protein (RBP) is a low-molecular-mass protein (21 kDa), easily filtered in renal glomeruli and very efficiently reabsorbed by the proximal convoluted tubules (PCTs). In PCT dysfunction, high concentrations of RBP are found in urine. Several methods have been used to determine RBP in serum or urine. We describe the production, selection, labeling, and utilization of anti-RBP monoclonal antibodies in two- or one-step immunoenzymometric assays for the determination of RBP. The one-step assay has good precision, with within-run and between- run CVs < 6.6% and 5.9%, respectively. Comparison with radial immunodiffusion (x) showed good agreement: y = 0.068 mg/L + 0.899x (n = 24). Comparison between the one-step (y) and two-step (x) versions of the assay also showed a very good correlation: y = 212 micrograms/L + 0.910x. The one-step assay has been adopted for routine work; it detects transthyretin-bound as well as free RBP and may have clinical usefulness in evaluating the functional status of PCTs.




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Copyright © 1993 by the American Association for Clinical Chemistry.