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Clinical Chemistry, Vol 39, 533-536, Copyright © 1993 by American Association for Clinical Chemistry
O Giampietro, G Penno, A Clerico, L Cruschelli and M Cecere
Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica 2a, Pisa, Italy.
We used three study protocols to check the dependence of albumin stability, measured by an RIA, on different temperatures, durations, and materials (i.e., assay tubes) of urine storage. Albumin values obtained for samples stored in three types of assay tubes (glass, polystyrene, and polypropylene) throughout the 2 months of the first (prospective) protocol were superimposable. The 24-h storage of six urine samples at room temperature or at 4 degrees C, as well as 72-h storage at 4 degrees C, did not affect the albumin measurement by RIA. After 2 months of storage of these same six urine samples at -20 degrees C, there was still no albumin decrease. A significant albumin decrease occurred (a mean of approximately 5% per year, throughout the range of albumin concentrations tested) when samples stored at -20 degrees C were reassayed by RIA after > or = 2 years (second protocol, retrospective). Finally, 3 of 21 (14.3%) urine pools stored at -20 degrees C for various periods (4-21 months) showed a significant albumin loss after storage; the time of storage as well the decrement rates of these 3 pools differed from each other (third protocol, retrospective). Short- and medium-term (2-6 months) freezing of urine samples at -20 degrees C does not significantly affect the stability of immunoreactive albumin. For longer preservation periods, storage of urine samples at -70 degrees C may be preferable.
The following articles in journals at HighWire Press have cited this article:
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  D. Sviridov, S. K. Drake, and G. L. Hortin Reactivity of Urinary Albumin (Microalbumin) Assays with Fragmented or Modified Albumin Clin. Chem., January 1, 2008; 54(1): 61 - 68. [Abstract] [Full Text] [PDF]
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J. W. Brinkman, D. de Zeeuw, H. J. Lambers Heerspink, R. T. Gansevoort, I. P. Kema, P. E. de Jong, and S. J.L. Bakker Apparent Loss of Urinary Albumin during Long-term Frozen Storage: HPLC vs Immunonephelometry Clin. Chem., August 1, 2007; 53(8): 1520 - 1526. [Abstract] [Full Text] [PDF]
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J. W. Brinkman, D. de Zeeuw, J. J. Duker, R. T. Gansevoort, I. P. Kema, H. L. Hillege, P. E. de Jong, and S. J.L. Bakker Falsely Low Urinary Albumin Concentrations after Prolonged Frozen Storage of Urine Samples Clin. Chem., November 1, 2005; 51(11): 2181 - 2183. [Full Text] [PDF]
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 M. Roest, J. D. Banga, W. M. T. Janssen, D. E. Grobbee, J. J. Sixma, P. E. de Jong, D. de Zeeuw, and Y. T. van der Schouw Excessive Urinary Albumin Levels Are Associated With Future Cardiovascular Mortality in Postmenopausal Women Circulation, June 26, 2001; 103(25): 3057 - 3061. [Abstract] [Full Text] [PDF]
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I. S. Klasen, L. J.M. Reichert, C. M. de Kat Angelino, and J. F.M. Wetzels Quantitative Determination of Low and High Molecular Weight Proteins in Human Urine: Influence of Temperature and Storage Time Clin. Chem., March 1, 1999; 45(3): 430 - 432. [Full Text] [PDF]
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V. T. Innanen, B. M. Groom, and F. M. de Campos Microalbumin and Freezing Clin. Chem., June 1, 1997; 43(6): 1093 - 1094. [Full Text] [PDF]
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