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Clinical Chemistry 39: 537-539, 1993;
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Clinical Chemistry, Vol 39, 537-539, Copyright © 1993 by American Association for Clinical Chemistry

Ion-chromatographic determination of plasma oxalate reexamined

M Petrarulo, E Cerelli, M Marangella, F Maglienti and F Linari
Renal Stone Laboratory, Mauriziano Hospital Umberto I, Turin, Italy.

This new procedure for determining oxalic acid in plasma is based on sample deproteinization with hydrochloric acid and acetonitrile and subsequent ion-chromatographic assay of the neutralized supernate. Sample pretreatment produces very clean samples, which ensures long column life. Mean analytical recovery of oxalate (5.0-10.0 mumol/L) added to plasma samples averaged 98.6 +/- 6.2%; imprecision (CV) was 5.2% (at 2.2 mumol/L) and the detection limit was 0.5 mumol/L at a signal-to-noise ratio of 5:1. Ascorbate to oxalate conversion was < 0.2%, indicating that the procedure is free from ascorbate interference. Plasma oxalate concentrations, measured in samples from 31 healthy persons, ranged from 0.8 to 3.4 mumol/L (mean 1.89, SD 0.75 mumol/L), which agrees with results from indirect radioisotopic dilution methods.


The following articles in journals at HighWire Press have cited this article:


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 Proc. Natl. Acad. Sci. USAHome page
E. C. Salido, X. M. Li, Y. Lu, X. Wang, A. Santana, N. Roy-Chowdhury, A. Torres, L. J. Shapiro, and J. Roy-Chowdhury
Alanine-glyoxylate aminotransferase-deficient mice, a model for primary hyperoxaluria that responds to adenoviral gene transfer
PNAS, November 28, 2006; 103(48): 18249 - 18254.
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Copyright © 1993 by the American Association for Clinical Chemistry.