Clinical Chemistry, Vol 39, 619-624, Copyright © 1993 by American Association for Clinical Chemistry

One-step competitive immunochromatographic assay for semiquantitative determination of lipoprotein(a) in plasma

SC Lou, C Patel, S Ching and J Gordon
Abbott Diagnostics Division, Abbott Laboratories, North Chicago, IL 60064-3500.

Numerous studies have associated high concentrations of lipoprotein(a) [Lp(a)] with atherosclerosis. We developed a rapid, one-step competitive immunochromatographic assay to measure Lp(a) in plasma. The assay is performed on a nitrocellulose membrane strip and the result is determined by a visual readout of rust-colored colloidal selenium. The assay is based on the principle that Lp(a) in the sample will compete with Lp(a)-coated colloidal selenium for binding to the anti-Lp(a) monoclonal antibody immobilized on the assay strip in the format of four ladder bars. The number of capture bars that appear as a result of the formation of colloidal selenium color is proportional to the concentration of the Lp(a) protein in the samples. The strip assay semiquantitatively measures Lp(a) concentrations ranging from 0 to 180 mg/L of Lp(a) protein in serum, plasma, or fingerstick whole-blood samples. This assay appears very useful for quick identification of individuals with above-normal concentrations of plasma Lp(a) protein (> 70 mg/L), and has potential for monitoring a patient's response to treatment with Lp(a)-lowering drugs.

The following articles in journals at HighWire Press have cited this article:

				A. H. Peruski and L. F. Peruski Jr. Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents Clin. Vaccine Immunol., July 1, 2003; 10(4): 506 - 513. [Full Text] [PDF]

				H.-s. Shin, C.-k. Kim, K.-s. Shin, H.-k. Chung, and T.-r. Heo Pretreatment of Whole Blood for Use in Immunochromatographic Assays for Hepatitis B Virus Surface Antigen Clin. Vaccine Immunol., January 1, 2001; 8(1): 9 - 13. [Abstract] [Full Text] [PDF]