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Clinical Chemistry 39: 686-689, 1993;
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Clinical Chemistry, Vol 39, 686-689, Copyright © 1993 by American Association for Clinical Chemistry

Automated spectrophotometric method for determining oxidized and reduced glutathione in liver

JP Teare, NA Punchard, JJ Powell, PJ Lumb, WD Mitchell and RP Thompson
Gastrointestional Laboratory, Rayne Institute, London, UK.

An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.




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